Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab)
CAT#: TAB-077
Recombinant monoclonal antibody to Integrin Integrin alpha 5 beta 1. Volociximab (also known as M200) is a chimeric monoclonal antibody jointly for treatment of a variety of advanced solid tumors. It binds to and inhibits the functional activity of α5β1 integrin.
It is thought to reduce metastases. Early results show potential in renal cell cancers.
Published Data
Gene Expression
Tested Data
IHC
Figure 1 Testing antibody cross-reactivity to human α5β1.
IIA1, the mouse parent antibody of volociximab, which recognizes only human integrin, anti-mouse CD31, which stains mouse vessels, and pooled rat IgG are shown as controls.
Bhaskar, V., Zhang, D., Fox, M., Seto, P., Wong, M. H., Wales, P. E., ... & Ramakrishnan, V. (2007). A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo. Journal of translational medicine, 5(1), 61.
ELISA
Figure 2 Representative antibodies were compared by ELISA for binding to human and murine α5β1-Fc or irrelevant Fc fusion proteins.
A subset of competitive antibodies cross-reacted with human integrin.
Bhaskar, V., Zhang, D., Fox, M., Seto, P., Wong, M. H., Wales, P. E., ... & Ramakrishnan, V. (2007). A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo. Journal of translational medicine, 5(1), 61.
ELISA
Figure 3 Binding of volociximab to integrin α5β1 and inhibition of integrin binding to fibronectin.
A dilution series of volociximab was incubated with immobilized human, mouse or rabbit integrin α5β1- Fc fusion proteins and bound volociximab was detected using an anti-human κ-chain specific antibody.
Bhaskar, V., Fox, M., Breinberg, D., Wong, M. H., Wales, P. E., Rhodes, S., ... & Ramakrishnan, V. (2008). Volociximab, a chimeric integrin alpha5beta1 antibody, inhibits the growth of VX2 tumors in rabbits. Investigational new drugs, 26(1), 7-12.
IHC
Figure 4 VX2 prevention study. Thirty rabbits were inoculated with subcutaneous tumors on the left hind limb.
Volociximab-treated rabbits generally grew fewer tumor-associated vessels than vehicle-treated rabbits
Bhaskar, V., Fox, M., Breinberg, D., Wong, M. H., Wales, P. E., Rhodes, S., ... & Ramakrishnan, V. (2008). Volociximab, a chimeric integrin alpha5beta1 antibody, inhibits the growth of VX2 tumors in rabbits. Investigational new drugs, 26(1), 7-12.
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Normal Tissue
Figure 1 High expression in placenta
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_B_1_6_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9008/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_8_4.jpg
Normal Tissue
Figure 5 Placenta
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: Low Intensity: Moderate Quantity: <25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_3_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000161638-ITGA5
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SDS-PAGE
Figure 1 Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody in SDS-PAGE analysis.
SDS-PAGE analysis of TAB-077 in reduced (lane 1) and non-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol- reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Chimeric (mouse/human)
- Type
- IgG4
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- ELISA, IHC
- CAS
- 558480-40-3
- Generic Name
- Volociximab
- UNII
- 496K5Z02NW
- MW
- 145.5 kDa
- Related Disease
- Unknown
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The Integrin alpha 5 beta 1 antibody has been reported in applications of Immunohistochemistry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Volociximab;558480-40-3;M200;ITGA5;integrin, alpha 5 (fibronectin receptor, alpha polypeptide);FNRA;integrin alpha-5;CD49e;VLA-5;integrin alpha-F;CD49 antigen-like family member E;fibronectin receptor subunit alpha;fibronectin receptor, alpha subunit;very
- Gene ID
- 3678
- UniProt ID
- P08648
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(Creative Biolabs Cat# TAB-077, RRID: AB_3111794)
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Biosimilar Overview
Please refer to Volociximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Volociximab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Volociximab"
Afuco™ Anti-ITGA5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-077)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Integrin Integrin alpha 5 beta 1. It is a chimeric monoclonal antibody jointly for treatment of a variety of advanced solid tumors. It binds to and inhibits the functional activity of α5β1 integrin.
It is thought to reduce metastases. Early results show potential in renal cell cancers.
DrugMonitor™ Anti-Volociximab Antibody (VS-1224-YC1312)Volociximab is a chimeric monoclonal antibody developed for treating various advanced solid tumors. The DrugMonitor™ Anti-Volociximab Antibody (VS-1224-YC1312) is an anti-drug antibody (ADA) against Volociximab. This drug-based antibody is raised in mice immunized with the Volociximab. The anti-Volociximab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Volociximab in samples.
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